Finally got around to prototyping the first of what will hopefully be a variety of useful objects created from waste materials, knitted together with mycelium, part of our larger efforts with biofabrication, bioprinting, and fermentation science. As our first mycelium project, we’re trying to make a 4″ planting pot that can be composted. We’ve got oyster mushroom jars rolling, ready to inoculate a variety of materials, including straw, rice hulls, coffee grounds (we worked out a deal with the coffee cart on campus to gather their grounds), waste cardboard, and hopefully various combinations of those things.

Mycelium!

The sample mushroom packaging material arrived last week, so we’ve gotten to touch and feel and get a sense of a commercial version of the material.

Mushroom Packaging

I created a model of the pot using Tinkercad, with the goal of 3D printing it in PLA and then using that model to create the form using the Formech Compaq Mini. The vacuum former tends to hold onto objects, so I designed the inside and outside walls to slope slightly, the outsides toward the middle – / \ – and the insides away from the middle – \ /, which I thought might make the plastic mold more likely to let go of the model. It didn’t quite work out that way, but more on that later. I also included a hole in the center, partly for drainage, and partly because I thought it would aid in the vacuum forming process.

Plant Pot Model

Hayes (student and Innovation Center staff) was kind enough to print the model to my specifications, which turned out to be wrong. More on that later. Anyhow, I asked for minimum infill, as the pot itself is 4″ at the base, and at least that tall, and I was interested in a quick print, rather than a durable one.

Printing the Pot

The model was ready this morning, so we set it up on the vacuum former.

Prototype In Place

It took a few rounds of heating, because we didn’t realize that the frame that holds the plastic down and creates the seal that allows the vacuum to form was out of alignment. Once we solved that problem, the process seemed to work really well, except…

V1 Mycopot Model

The repeated heating, coupled with my desire for a fast print rather than a strong one, added up to a mistake. Specifically, the PLA model melted and warped – you can see the jankiness above – and as a consequence, the model stuck in the deformed plastic sheet, and I had to pull it apart layer by layer to get it release.

V1 Mycopot Model Melted

Even with the less than perfect walls, the form is more or less usable, but we’re going to print a much more solid version in PLA on the Ultimakers, and a more solid version using the Form2 and maybe the tough resin. We learned a lot from the process, which is the beauty of prototyping!

MUSHROOMS!

Babies

Inspired by projects like Ecovative’s building and packaging materials – check out this guide to How to Make Your Own Growth Forms – and in line with our other biotinkering and fermentation science efforts, we’ve been slowly gathering mushroom making gear, including an autoclave…

Pressure

and a laminar flow hood.

Hoodie

The liquid mushroom culture syringes arrived, so we inoculated some sterile rye berry jars.

Innoculated

With any luck, the jars will take, and we’ll be able to begin mass production. Meanwhile, we’re figuring out our new Formech vacuum former, and we think there are opportunities to use it in conjunction with our 3D printers and CNC machines to create custom forms for growing mushrooms in the makerspace.

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