Finally got around to prototyping the first of what will hopefully be a variety of useful objects created from waste materials, knitted together with mycelium, part of our larger efforts with biofabrication, bioprinting, and fermentation science. As our first mycelium project, we’re trying to make a 4″ planting pot that can be composted. We’ve got oyster mushroom jars rolling, ready to inoculate a variety of materials, including straw, rice hulls, coffee grounds (we worked out a deal with the coffee cart on campus to gather their grounds), waste cardboard, and hopefully various combinations of those things.

Mycelium!

The sample mushroom packaging material arrived last week, so we’ve gotten to touch and feel and get a sense of a commercial version of the material.

Mushroom Packaging

I created a model of the pot using Tinkercad, with the goal of 3D printing it in PLA and then using that model to create the form using the Formech Compaq Mini. The vacuum former tends to hold onto objects, so I designed the inside and outside walls to slope slightly, the outsides toward the middle – / \ – and the insides away from the middle – \ /, which I thought might make the plastic mold more likely to let go of the model. It didn’t quite work out that way, but more on that later. I also included a hole in the center, partly for drainage, and partly because I thought it would aid in the vacuum forming process.

Plant Pot Model

Hayes (student and Innovation Center staff) was kind enough to print the model to my specifications, which turned out to be wrong. More on that later. Anyhow, I asked for minimum infill, as the pot itself is 4″ at the base, and at least that tall, and I was interested in a quick print, rather than a durable one.

Printing the Pot

The model was ready this morning, so we set it up on the vacuum former.

Prototype In Place

It took a few rounds of heating, because we didn’t realize that the frame that holds the plastic down and creates the seal that allows the vacuum to form was out of alignment. Once we solved that problem, the process seemed to work really well, except…

V1 Mycopot Model

The repeated heating, coupled with my desire for a fast print rather than a strong one, added up to a mistake. Specifically, the PLA model melted and warped – you can see the jankiness above – and as a consequence, the model stuck in the deformed plastic sheet, and I had to pull it apart layer by layer to get it release.

V1 Mycopot Model Melted

Even with the less than perfect walls, the form is more or less usable, but we’re going to print a much more solid version in PLA on the Ultimakers, and a more solid version using the Form2 and maybe the tough resin. We learned a lot from the process, which is the beauty of prototyping!

This semester, Jennifer Kraemer (professor of Early Childhood Education and makerspace champion) and I are teaching a new course we developed – Making For Educators – for the first time, a hands-on course to help educators incorporate making into their teaching practice. It’s the second of our discipline-lensed making courses, including Making Social Change, a Sociology course we’ve taught now twice, in which students explore social movements and the ways that those movements use tools to enact change. For ECE 452, we’re using the excellent Invent to Learn by Sylvia Libow Martinez (@smartinez) and Gary Stager, Ph. D. (@garystager) as our text, and the class meets in the makerspace.

Tinkering Night in ECE 452 - Making for Educators

Our most recent class session was focused on tinkering. Martinez and Stager define tinkering thus:

Tinkering is a mindset – a playful way to approach and solve problems through direct experience, iteration, experimentation, and discovery.

At the start of the class, we talked about the relationship between tinkering, making and engineering, shared some examples, and after a very brief discussion of safety – no plugging things in! – students chose an item from a pile of broken and obsolete printers, laptops and desktop computers, tape and optical drives, and other electronic detritus, and began the process of unscrewing cases, cutting cables, and generally deconstructing their items.

Tinkering Night in ECE 452 - Making for Educators

Most students worked independently, content to explore the guts of their chosen electronic devices, but a pair of students decided to work together, taking apart a tape backup drive…

Tinkering Night in ECE 452 - Making for Educators

and knolling the parts.

Knolled

Students settled quickly into the work, and were laughing and sharing and generally having a good time. Unsurprisingly perhaps, many reported never having seen the inside of a laptop or DVD drive, and they were excited to explore. At the end of the session, we asked them to share their thoughts and feelings as they tinkered.

Thoughts & Feelings While Tinkering

Students shared feelings of pride and satisfaction, and described the activity as therapeutic, relaxing, savage, confusing, and cathartic. After almost two years of development, it’s satisfying (and therapeutic, relaxing, savage, confusing, and cathartic) to get this class up and running, and we’re looking forward to learning alongside these brave and creative students.

Toward the end of last semester – after lengthy and vigorous and unflinching hacking of red tape – we offered the first workshop – Beer Science: Measuring Beer Bitterness – as part of our ongoing Fermentation Science efforts. We started the day in the Chemistry lab, where Max Mahoney (Chemistry professor and makerspace faculty champion) described the chemistry of beer, and led students through a procedure for measuring beer bitterness.

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Here’s how Max describes it:

The goal of this workshop was to expose students to a quantitative and qualitative analysis of beer bitterness. The chemistry of hops and bittering compounds was presented along with a discussion of the chemical procedures involved in this analysis. The following procedure was used to quantitatively analyze beer bitterness. Three beers were selected containing different levels of the hop-derived bittering agents. Students sonicated the beer to expel carbon dioxide, performed a liquid-liquid extraction of the hop acids with iso-octane, and measured the UV and visible absorption spectrum for their sample. We used the visible absorption spectra to help classify the style of beer. The UV absorption was used to quantify the concentration of hop acids and thus the bitterness of the beer (measured in IBUs).

Chemistry students of all levels were able to learn advanced analytical methods used in the beverage industry to analyze beer bitterness. General and organic chemistry lab techniques were utilized including UV-Vis spectroscopy, usage of micropipettes, and liquid-liquid extraction of organic compounds.

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The Chemistry lab portion completed, we went over to the Innovation Center for some blind taste tests. Students sampled various beers, and then used PollEverywhere to report the perceived bitterness of the sample, the results of which we compared to the lab-derived values.

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The event was a terrific success, and students were engaged and enthusiastic. We’ve got additional interdisciplinary FermSci workshops and projects planned for this semester, including more beer chemistry, sauerkraut making, curriculum development, and a partnership with a local employer for integrating IOT technology into kombucha fermentation.

The new semester has started, and things seem to be happening at a furious pace.

As part of professional development days preceding the semester, we invited faculty and staff for a makerspace update, and facilitated a prototyping workshop, solving problems having to do with babies and robots.

Flex Workshop Prototyping

Our staff did some outreach to invite students to participate in our community.

Falcons_Day

Also on the community front, we participated (for the third time) in the third annual Georgetown School Family Tinker Night, an event coordinated by our sister lab at Georgetown School. We brought out a 3D bioprinter and a plotter, and the ever popular Nova (our space bunny mascot) fresnel lens family face distorters.

Nova Faces

Meanwhile, back in the lab…

The jars we inoculated with mushroom spawn earlier in the month are thriving, and we’ll be scaling that project up soon.

Mycelium!

The Science Fish have returned from the library, as we plan and implement whatever v2.0 of our aquaponics efforts will look like. Yes, those are the same three fish – Phoebe, Phinley, and Phreud – still with us after more than two years, and yes, the water cleared up quickly and it’s crystal clear now.

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As part of the CCC Maker grant, we’re able to pay interns to do makerspace-related projects, and some of them are working on a large-scale, interactive periodic table of the elements, to be installed in our large lecture hall. Here Max Mahoney (Chemistry) and Nicole (makerspace facilitator extraordinaire) review some prototypes.

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We’re looking forward to starting the semester with a Grand Opening next week, and to continuing to advance various efforts, including FermSci and biotinkering, some salon-type events in the planning stages, and a million other things I’m probably forgetting. Onward and upward.

MUSHROOMS!

Babies

Inspired by projects like Ecovative’s building and packaging materials – check out this guide to How to Make Your Own Growth Forms – and in line with our other biotinkering and fermentation science efforts, we’ve been slowly gathering mushroom making gear, including an autoclave…

Pressure

and a laminar flow hood.

Hoodie

The liquid mushroom culture syringes arrived, so we inoculated some sterile rye berry jars.

Innoculated

With any luck, the jars will take, and we’ll be able to begin mass production. Meanwhile, we’re figuring out our new Formech vacuum former, and we think there are opportunities to use it in conjunction with our 3D printers and CNC machines to create custom forms for growing mushrooms in the makerspace.

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We recently unboxed our SE3D r3bEL mini bioprinter, which we plan to use for research and development aligned with our fermentation science and other biotinkering efforts.

Bioprinter Arrives!

After some initial setup, I realized that the build plate meant to house petri dishes didn’t fit our petri dishes, so I contacted SE3D and asked for a vector file of the shipped build plates so as to modify one. While waiting for the email back, I went ahead and just measured the existing one, and after a few prototypes, I was able to cut a new one to the right size and shape out of acrylic. In the meantime, Vignesh got back to me – they’re very responsive! – with the DXF file of the build plates that arrived with the machine.

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With the petri dish sorted, I set out to print the stock test file. So far, so good.

3D Printed Bone

A successful test completed, I found a *.stl file of Nova (our Innovation Center mascot and the thing we traditionally create using any new machine), imported it into Slic3r, exported as G-code, and fired up the r3bEL. Other than the fact that the syringe ran out of lotion before the print was finished, it worked a treat!

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I feel fairly confident in saying that this might be the first time in the history of the world that a rabbit wearing a space helmet was 3D printed out of lotion. #fiteme

You Are Here

We are here.  Diane Carlson (Sociology) and I are co-teaching v2.0 of Making Social Change, a hands-on course at the intersection of making and Sociology, in which we explore social movements and the ways that they use tools to enact change. We teach the class in the makerspace, and we’re working with a brave cohort of interesting students. We’ve been tweaking and adapting the content, activities, and flow, building on what we learned offering a prototype of the course in fall of 2017.

So far, we’ve spoken with Ivan in South Africa, a friend of Diane’s and an ANC activist who fought against apartheid…

Talking to a South African Activist

…worked with the laser cutter and 3D printer to create a Harriet Tubman stamp to perfect a twenty dollar bill…

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…and discussed memory and monument, working through James W. Loewen’s ideas in Lies Across America

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…and creating prototypes for potential monuments to be built on our college campus.

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But just a little bit.

Took the crocheted SCOBY mat home to dry it out, and kept it overnight in a 120° F oven on parchment paper to dry it out.

SCOBY Mat

The thicker side didn’t quite incorporate itself with the hemp fiber, owing I think to the fact that the crocheted piece was suspended slightly below the high tea/sugar mark.

Microcellulose and Cellulose

In some places though, it seems as though the SCOBY really integrated with the mat.

Texture

The finished piece has an interesting flexibility because of the crocheted core.

Light Emitting

I decided to try using SNO-SEAL, which is beeswax and some sort of solvent, to waterproof the mat. I melted some in an old pan on an outdoor stove, and plunged the SCOBY mat into it.

Structure

As I was turning off the flame, the pan caught fire, which was no big deal, and I extinguished it by putting a piece of metal over it. I didn’t realize, however that the mat itself was on fire, but was able to put it out quickly. I then placed the whole business on parchment paper and into the oven at 120° again for about 20 minutes, then buffed it out with some paper towels.

Beeswaxed

It definitely sheds water.

Our next experiments include creating magnetic SCOBY by incorporating iron filings into some fruit leather mush, and creating “leather” bracers for an upcoming fashion show.

Fiber Woven SCOBY

Decided that the crocheted SCOBY was ready for drying, so removed it from its vessel…

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…washed and rinsed it…

To Dry

…and set it out in the sun to dry.

Parchment Paper in the Sun

In some places, it’s really well integrated with the hemp twine mat, and so we’re curious to see how it all looks and acts, and what effect the hemp fibers have on the finished piece.

Ceulluse Integration

SCOBY Under the Microscope

Max (Chemistry professor and makerspace champion) has a nice oil immersion scope that hadn’t been used in years, so we fired it up, borrowed some oil, and looked at some of the SCOBY pellicle, both the mashed variety and the paper thin one.

Nicole Scopin'

The scope needs a good cleaning, but Jared, Nicole, and Brett (makerspace students and staff) were able to get a view into the microscopic world of kombucha. The images above were all shot with mobile phones through the eyepiece, but we’re excited to use one of the scopes in the Biology labs with the built-in cameras.

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